期刊
INTERNATIONAL JOURNAL OF IMMUNOPATHOLOGY AND PHARMACOLOGY
卷 34, 期 -, 页码 -出版社
SAGE PUBLICATIONS INC
DOI: 10.1177/2058738420950593
关键词
acute liver injury; carbon tetrachloride; mitochondrial reactive oxygen species; NLRP3 inflammasome; oxidative stress; saikosaponin-d
资金
- National Natural Science Foundation of China [81573775, 81873157]
- Natural Science Foundation of Shanghai [09ZR1429700]
- Outstanding Academic Leader of Health System of Shanghai [XBR2013120]
- Youth Talent Training Plan of Shanghai Municipal Hospital of Traditional Chinese Medicine [2019PY003]
- budgetary Foundation of Shanghai University of Traditional Chinese Medicine [18LK074]
- Shanghai Sailing Program [19YF1445200]
NLRP3 inflammasome activation results in severe liver inflammation and injury. Saikosaponin-d (SSd) possesses anti-inflammatory and hepatoprotective effects. This study aimed to determine the protective effects of SSd on carbon tetrachloride (CCl4)-induced acute liver injury in mice, and whetheroxidative stress and NLRP3 inflammasome activation participate in the process. The CCl(4)mice model and controls were induced. The mice were treated with SSd at 1, 1.5, or 2.0 mg/kg in a total volume of 100 mu l/25 g of body weight. Liver injury was assessed by histopathology. Oxidative stress was determined using mitochondrial superoxide production (MSP), malondialdehyde (MDA) content, and superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT) activities. NLRP3, ASC, and Caspase 1 were determined by real-time PCR and western blot. IL-1 beta and IL-18 levels were determined by ELISA. Significantly elevated oxidative stress was induced in the liver by CCl4, as demonstrated by histopathology and increases of MDA and MSP levels and decreases of SOD, GPx, and CAT activities (allP < 0.01). SSd significantly decreased the MDA and MSP levels and increased the activities of SOD, GPx, and CAT (allP < 0.05). The mRNA expression of NLRP3, ASC, and Caspase 1, and the protein expression of Caspase 1-p10, NLRP3, ASC, IL-1 beta, and IL-18 were significantly increased after CCl(4)induction (allP < 0.01). These changes were reversed by SSd (allP < 0.05). Suppression of the oxidative stress and NLRP3 inflammasome activation were involved in SSd-alleviated acute liver injury in CCl4-induced hepatitis.
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