期刊
INSECT MOLECULAR BIOLOGY
卷 26, 期 6, 页码 677-686出版社
WILEY
DOI: 10.1111/imb.12327
关键词
Dusky; Four-jointed; Forked; wing; Tribolium castaneum
资金
- China Postdoctoral Science Foundation [2015M581729]
- National Natural Science Foundation of China [31601893, 31572467, 31572326]
- Natural Science Foundation of the Jiangsu Higher Education Institutions [16KJB180004]
- Natural Science Foundation of Jiangsu Province [BK20150495]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
Dusky (dy) is required for cytoskeletal reorganization during wing morphogenesis in Drosophila melanogaster, but which genes participate together with dy for wing morphogenesis has remained unclear. In Tribolium castaneum, dy is highly expressed at the late embryonic stage. Tissue-specific expression analysis indicated high expression levels of dy in the epidermis, head and fat body of late-stage larvae. RNA interference (RNAi) targeting dy significantly decreased adult wing size and caused improper folding of the elytra. Meanwhile, dy knockdown reduced the transcription of four-jointed (fj) and forked (f). Our results show that fj RNAi reduces adult wing size and that silencing f results in abnormal wing folding in T.castaneum. Interestingly, knocking down fj and f simultaneously phenocopies dy RNAi, suggesting that dy probably acts upstream of fj and f to regulate wing morphogenesis in T.castaneum.
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