期刊
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
卷 88, 期 -, 页码 30-36出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.ibmb.2017.07.007
关键词
Chloroquine; Aversive behavior; Electrophysiology; GR22e; GR33a
资金
- Global Scholarship Program for Foreign Graduate Students at Kookmin University in Korea
- Basic Science Research Program of the National Research Foundation of Korea (NRF) - Ministry of Education [2014R1A1A2058094, 2016R1D1A1B03931273]
- National Research Foundation of Korea [2016R1D1A1B03931273, 2014R1A1A2058094] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Chloroquine, an amino quinolone derivative commonly used as an anti-malarial drug, is known to impart an unpleasant taste. Little research has been done to study chloroquine taste in insects, therefore, we examined both the deterrant properties and mechanisms underlying chloroquine perception in fruit flies. We identified the antifeedant effect of chloroquine by screening 21 gustatory receptor (Grs) mutants through behavioral feeding assays and electrophysiology experiments. We discovered that two molecular sensors, GR22e and GR33a, act as chloroquine receptors, and found that chloroquine-mediated activation of GRNs occurs through S-type sensilla. At the same time, we successfully recapitulated the chloroquine receptor by expressing GR22e in ectopic gustatory receptor neurons. We also found that GR22e forms a part of the strychnine receptor. We suggest that the Drosophila strychnine receptor might have a very complex structure since five different GRs are required for strychnine-induced action potentials. (C) 2017 Elsevier Ltd. All rights reserved.
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