期刊
INORGANIC CHEMISTRY
卷 56, 期 21, 页码 13293-13299出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.inorgchem.7b02073
关键词
-
资金
- European Research Council (ERC) [280010]
- Netherlands Organisation for Scientific Research [724.013.003]
- Ministry of Education, Culture and Science (Gravity programme) [024.001.035]
- China Scholarship Council (CSC)
The interaction of a number of first-row transition-metal ions with a 2,2'-bipyridyl alanine (bpyA) unit incorporated into the lactococcal multidrug resistance regulator (LmrR) scaffold is reported. The composition of the active site is shown to influence binding affinities. In the case of Fe(II), we demonstrate the need of additional ligating residues, in particular those containing carboxylate groups, in the vicinity of the binding site. Moreover, stabilization of di-tert-butylsemiquinone radical (DTB-SQ) in water was achieved by binding to the designed metalloproteins, which resulted in the radical being shielded from the aqueous environment. This allowed the first characterization of the radical semiquinone in water by resonance Raman spectroscopy.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据