期刊
INJURY-INTERNATIONAL JOURNAL OF THE CARE OF THE INJURED
卷 48, 期 7, 页码 1480-1485出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.injury.2017.05.015
关键词
Nerve regeneration; BMMCs; Chitosan conduit
Background: Employment of regenerative properties of cells at the service of nerve repair has been initiated during recent decades. Effects of local transplantation of bone marrow-derived mast cells on peripheral nerve regeneration were studied using a rat sciatic nerve transection model. Materials and methods: A 10-mm sciatic nerve defect was bridged using a conduit chitosan-based hybrid conduit filled with BMMCs in BMMC group. In positive control group (Pos), the conduit was filled with phosphate-buffered saline alone. The regenerated nerve fibers were studied within 12 weeks after surgery. In sham-operated group, the sciatic nerve was only exposed and manipulated. In negative control (Neg) a 10-mm sciatic nerve defect was created and the nerve stumps were sutured to the adjacent muscles. The regenerated nerve fibers were studied functionally, biomechanically, histologically and immunohiscochemically. Results: Functional and biomechanical studies confirmed faster recovery of regenerated axons in BMMCs transplanted animals compared to Pos group (p < 0.05). Morphometric indices of the regenerated fibers showed that the number and diameter of the myelinated fibers were significantly higher in BMMCs transplanted animals than in Pos group (p < 0.05). In immunohistochemistry, location of reactions to S100 in BMMCs transplanted animals was clearly more positive than that in Pos group. Conclusions: BMMCs transplantation could be considered as a readily accessible source of cells that could improve functional recovery of transected sciatic nerve. (C) 2017 Elsevier Ltd. All rights reserved.
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