期刊
CHEMOSPHERE
卷 258, 期 -, 页码 -出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.chemosphere.2020.127332
关键词
Raw water; Reporter gene assays; Endocrine disrupting activities; Aryl hydrocarbon receptor; Steroid receptors; Benzo[a]pyrene
资金
- European Union's Horizon 2020 research and innovation program under the Marie Sklodowska-Curie grant [722634]
Prioritizing chemicals posing threats to drinking water resources is crucial for legislation considering the cost of water treatment, remediation, and monitoring. We profiled in vitro potential endocrine disrupting activities (both agonistic and antagonistic) of 18 contaminants most prevalent in Walloon raw water resources intended for drinking water production, including several compound groups: pesticides, perfluorinated compounds, polycyclic aromatic hydrocarbons, a corrosion inhibitor, and bisphenol A. Mixtures thereof relevant for human realistic exposure were also investigated. Seven luciferase reporter gene cell lines were used i.e. three (human and rat) responsive to dioxins through the aryl hydrocarbon receptor (AhR) and four (human) responsive to steroids through the estrogen (ER), androgen (AR), progesterone (PR), and glucocorticoid (GR) receptors. Among the 18 compounds, ten caused at least one response in at least one receptor. Specifically, chlorpyrifos, bisphenol A, fluoranthene, phenanthrene, and benzo [a]pyrene displayed significant activities on several receptors. Bisphenol A agonized ER, but abolished the cells' response to androgen and progesterone. While fluoranthene and phenanthrene strongly reduced human AhR and AR transactivation, benzo [a]pyrene strongly activated AhR and ER, but inhibited GR and AR. In human breast cancer cells, benzo [a]pyrene dramatically activated AhR, inducing a 10-fold higher response than 2,3,7,8-tetrachlorodibenzodioxin (TCDD) at concentrations possibly found realistically in human blood. The mixture of the 18 compounds exerted both ER and rat AhR agonism, with the main contribution being from benzo [a]pyrene or its combination with bisphenol A. Moreover, the mixture significantly inhibited TCDD-induced CYPIA activity (detected only by EROD assays) in human hepatoma cells. (C) 2020 Elsevier Ltd. All rights reserved.
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