期刊
CANCER REPORTS
卷 3, 期 6, 页码 -出版社
WILEY
DOI: 10.1002/cnr2.1281
关键词
drug delivery; drug-resistant cells; hyperthermia; magnetic nanofibers; skin cancer
类别
资金
- Department of Biotechnology, Ministry of Science and Technology
- Department of Science and Technology, Ministry of Science and Technology
- INSPIRE Faculty Award, FIST
- University Grants Commission
Background The treatment of non-melanoma skin cancer and deadliest malignant melanoma skin cancer are the fifth and ninth most expensive treatments in Medicare, respectively. Moreover, the recurrence of cancer after currently available therapies, that is, surgery or radiotherapy, reduces the patient's life expectancy. Aims In view of this, we fabricated magnetic nanofibrous mat-based bandage to treat skin cancer non-invasively using an external alternating current (AC) magnetic field induced hyperthermia. Methods The Fe(3)O(4)nanoparticles incorporated polycaprolactone (PCL) fibers based bandages were fabricated using the electrospinning technique. The efficacy of the bandage was investigated in vitro using parental/doxorubicin hydrochloride (Dox)-resistant HeLa cells and in vivo using BALB/c mouse model in the presence of an external AC magnetic field (AMF). Results The PCL-Fe(3)O(4)fibrous mat-based bandages dissipate heat energy locally on the application of an external AMF and increase the surrounding temperature in a controlled way up to 45 degrees C in a few mins. The in vitro study confirms the elevated temperature could kill parental and Dox-resistant HeLa cells significantly. As the activity of Dox enhanced at a higher temperatures, more than 85% of parental HeLa cells were dead when cells incubated with Dox contained fibrous mat in the presence of AMF for 10 minutes. Further, we confirm the full recovery of chemically induced skin tumors on BALB/c mice within a month after five hyperthermic doses for 15 minutes. Also, there was no sign of inflammation and recurrence of cancer post-therapy. Conclusion The present study confirms the PCL-Fe(3)O(4)nanofibrous based bandages are unique and compelling to treat skin cancer.
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