Effects of aging and transformation of anatase and rutile TiO2 nanoparticles on biological phosphorus removal in sequencing batch reactors and related toxic mechanisms

The effect of nanomaterials aging, namely the transformation of comprehensive characteristics after experiencing real or complex environmental behaviors, on their ecotoxicology is still lacking. Moreover, the mechanisms by which NPs influence biological phosphorus (P) removal during sewage treatment require further elucidation. Therefore, we used both pristine and aged anatase (TiO2-A) and rutile (TiO2-R) NPs to investigate the mechanisms by which NPs affect P removal in a SBR. At 0.1 mg/L, the four types of NPs (pristine and aged) had no significant effect on sludge purification after acute (72-h) exposure under simulated sunlight. However, at 50 mg/L-regardless of the crystalline phase of the NPs-SOP and COD removal efficiency dropped steeply to approximately 42.2-82.4 % (p < 0.05) and 69.8-83.3 % (p < 0.05), respectively, especially in the pristine TiO2-NPs groups because of decrease of richness and diversity of genus level of PAOs and enzyme activity of both PPK and PPX, and the sluggish transformation of PHA and glycogen. Aging reduced the ability of NPs toxicity. The toxicity mechanisms of TiO2-NPs included lipid peroxidation and contact damage, or leakage from bacterial cytoplasmic membrane, which are closely related to photooxidation capacity and aqueous solution stability-i.e., nanoscale effects-and the impacts of aging or inclusion.