期刊
JOURNAL OF MOLECULAR STRUCTURE
卷 1219, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.molstruc.2020.128600
关键词
D-glutamine; DL-glutamic acid; Chemoenzymatic synthesis; L-aminopeptidase
资金
- National Key R&D Program of China [2017YFC0506005]
D-glutamine is a D type stereoisomer of glutamine which is involved in many metabolic processes. Seeking lower-cost and industrially scalable approaches for the synthesis of D-glutamine is very valuable both in academic career and potential applications. Herein, we developed a novel efficient chemoenzymatic strategy for producing D-glutamine. Initially, DL-glutamine was chemically prepared with cheap and accessible DL-glutamic acid as raw material. Subsequently, the L-glutamine among the racemic mixture was selectively hydrolyzed to L-glutamic acid by Escherichia coli whole-cell system which expressed L-aminopeptidase D-Ala-esterase/amidase (DmpA) from Ochrobactrum anthropi. The left D-glutamine was obtained by isoelectric point precipitation with 70% of the theoretical yield. Furthermore, we optimized enzymatic resolution conditions to determine the optimum parameters as pH 8, 30 degrees C, 0.1% (v/v) Triton X-10 0, and 1 mM Mn-2 thorn . These results suggested that our strategy might be potentially usable for the synthesis of D-glutamine in industrial productions. (C) 2020 Elsevier B.V. All rights reserved.
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