期刊
JOURNAL OF ETHNOPHARMACOLOGY
卷 262, 期 -, 页码 -出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.jep.2020.113211
关键词
Zuojin pill; Ulcerative colitis; Gut microbiota; Treg cells; PI3K/Akt signaling pathway
资金
- National Natural Science Foundation of China [81803988, 8180792, 81760838, 81760808]
- Natural Science Foundation of Jiangxi Province [20192ACB20015, 20192BAB215050, 20181BAB205082]
- Education Department of Jiangxi Province [GJJ171542]
- Jiangxi University of Traditional Chinese Medicine 1050 Young Talents Project [1141900603]
- First-Class Subjects Starting Funds [JXSYLXK-ZHYI022, JXSYLXK-ZHYAO132, JXSYLXK-ZHYAO108]
Ethnopharmacological relevance: As a classic prescription and commercial Chinese patent medicine, Zuojin Pill (ZJP) has been used to treat ulcerative colitis (UC) effectively for many years. However, its mechanism of action remains unclear. Aim of the study: Methods: Mice with dextran-sulfate-sodium-induced colitis were treated with ZJP for 7 d. In the present study, the therapeutic effect of ZJP was evaluated by macroscopic and microscopic observation; regulatory T (Treg) cells and their subsets were analyzed by flow cytometry; and the composition of gut microbiota was tested by 16S rRNA analysis. Activation of the phosphoinostide 3-kinase (PI3K)/Akt signaling pathway was observed by western blotting. Results: The pathological damage was attenuated and expression of proinflammatory cytokines was decreased. While the diversity of intestinal microflora was regulated, the relative abundance of Actinobacteria, and Sphingobacteriia was modified. Meanwhile, the level of CD4(+) CD25(+) Foxp3(+) and PD-L1(+) Treg cells improved. These changes maintained a positive correlation which was analyzed statistically. Our results also showed that ZJP inhibited activation of the PI3K/Akt signaling pathway. Conclusions: ZJP regulates crosstalk between intestinal microflora and Treg cells to attenuate experimental colitis via the PI3K/Akt signaling pathway.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据