期刊
INFECTION GENETICS AND EVOLUTION
卷 49, 期 -, 页码 256-267出版社
ELSEVIER
DOI: 10.1016/j.meegid.2017.01.029
关键词
Primary sheep testicular cells; Bluetongue virus; RNA sequencing; microRNAs
资金
- National Natural Science Foundation of China [31672562]
- Gansu International Collaboration Special Project [1504WKCA056]
- China-South Africa Joint Research Project [CS08-L13]
- Foundation for Returned Overseas Chinese Scholars, MOE
- ASTIP, CAAS
- NBCIS [CARS-38]
- Special Fund for Agro-scientific Research in the Public Interest, MOA [201303035]
- Jiangsu Co-innovation Center Program for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses
- State Key Laboratory of Veterinary Etiological Biology Project
Bluetongue virus (BTV) is a member of the genus Orbivirus within the family Reoviridae and causes a non-contagious, insect-transmitted disease in domestic and wild ruminants, mainly in sheep and occasionally in cattle and some species of deer. Virus infection can trigger the changes of the cellular microRNA (miRNA) expression profile, which play important post-transcriptional regulatory roles in gene expression and can greatly influence viral replication and pathogenesis. Here, we employed deep sequencing technology to determine which cellular miRNAs were differentially expressed in primary sheep testicular (ST) cells infected with BTV. A total of 25 known miRNAs and 240 novel miRNA candidates that were differentially expressed in BTV-infected and uninfected ST cells were identified, and 251 and 8428 predicted target genes were annotated, respectively. Nine differentially expressed miRNAs and their mRNA targets were validated by quantitative reverse transcription-polymerase chain reaction. Targets prediction and functional analysis of these regulated miRNAs revealed significant enrichment for several signaling pathways including MAPK, PI3K-Akt, endocytosis, Hippo, NF-kB, viral carcinogenesis, FoxO, and JAK-STAT signaling pathways. This study provides a valuable basis for further investigation on the roles of miRNAs in BTV replication and pathogenesis. (C) 2017 Elsevier B.V. All rights reserved.
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