期刊
LAB ON A CHIP
卷 20, 期 18, 页码 3346-3353出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/d0lc00432d
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资金
- National Research Foundation of Korea (NRF) - Korean government (MSIT) [NRF-2019R1A2B5B03070494, NRF-2017M3A7B4039936]
- National Research Foundation of Korea [4199990914370] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Molecular diagnostics can provide a powerful diagnostic tool since it can detect pathogens with high sensitivity, but complicated sample preparation procedures limit its widespread use as an on-site detection tool that relies on the skilled person and external equipment. To resolve these limitations, we report a solid-phase nucleic acid purification using a finger-actuated microfluidic device, which can control a set amount of flow regardless of differences in end-users. To increase the recovery rate, a finger-actuated reciprocator was newly developed and integrated into the microfluidic device that can efficiently react with silica microbeads and reagents. After verifying the finger-actuated microfluidic reciprocator, the effect of the reciprocating flow on the recovery rate was assessed to purify the standard DNA of the hepatitis B virus (HBV). The recovery rate was increased up to similar to 50% and 955 to 955 000 IU mL(-1)of HBV standard DNA was successfully purified and detected by a real-time polymerase chain reaction. Furthermore, the proposed microfluidic device was exploited to purify the HBV DNA from the patient's blood plasma samples.
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