期刊
INDUSTRIAL CROPS AND PRODUCTS
卷 107, 期 -, 页码 360-369出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.indcrop.2017.05.050
关键词
sugarcane; bagasse; recovery; phenolics; antioxidation; antiproliferation
资金
- National Natural Science Foundation of China [31301506]
- Guangdong Science and Technology Program [20148090904063]
- Water Resource Science and Technology Innovation Program of Guangdong Province [201624]
- Leading Talent of Guangdong Province
In this work, recovery of phenolics from sugarcane bagasse was performed and total phenolic content, total flavonoid content and antioxidant activities were measured. Four fractions were yielded after extraction with various solvents and ethyl acetate fraction was performed into further purification, producing 30 sub-fractions (G1-G30). Six compounds were isolated and their structures were identified by high resolution mass spectrometry (HR-MS) and high definition nuclear magnetic resonance (HD-NMR) spectroscopy as p-coumaric acid, tricin, luteolin, tricin 7-O-beta-glucopyranoside, protocatechuic add and diosmetin 6-C-glucoside which was identified in this species for the first time. Tricin expressed highest antioxidant activity in ORAC assay (9.76 +/- 1.01 mu mol TE/mu mol) but no antioxidant activity in PSC and CAA assays. The structure-activity relationship was discussed to elucidate the different activities of the isolated phenolics in ORAL and CAA assays. Luteolin, p-coumaric acid and protocatechuic acid showed antiproliferative effect against MCF-7 cells with the EC50 at 28.45, 1856.90 and 3670.56 mu M, respectively. This work reported the recovery of individual phenolics from bagasse, which suggested bagasse could be an abundant source of bioactive phenolics.
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