Marigold extract, azelaic acid and black caraway oil into lipid nanocarriers provides a strong anti-inflammatory effect in vivo

Therapeutic benefits of phytochemicals are widespread, but the association of natural lipophilic and hydrophilic compounds in the same nanostructured delivery system represents a challenge that was not considered up to present. This work aimed to evaluate the effects of two plant-derived oils rich in omega-3 (rosehip oil Ro) and omega-6 (black caraway oil Bco) on the synthesis of marigold extract (ME) and azelaic acid (AzA) co -loaded nanostructured lipid carriers(NLC) and to determine its in vitro and in vivo anti-inflammatory action. The lipid core and surfactant shell guaranteed a high encapsulation efficiency of both water soluble -AM and lipid soluble -ME(93% entrapment for carotenoids and 83% for AM). A high degree of biocompatibility in the concentration range of 5-400 mu g/mL NLC-ME-AzA for 72 h of treatment was determined (i.e. at 400 mu g/mL the L929 cells displayed a proliferative effect with a cell viability of 110%). The co-presence of ME and AM in the same NLC was accompanied by a reduced expression of inflammatory IL-6 and IL-1 beta cytokines, as evidenced by ELISA method. The in vivo plestismometric method showed that paw edema was significantly reduced for 3 h following NLC-ME-AzA hydrogel treatment (0.006 mg ME and 26.2 mg AAz) in rats challenged with carrageenan.