Two new triterpenoid saponins from the leaves of Impatiens parviflora DC. and their cytotoxic activity

Two new triterpene saponins, camelliagenin A derivatives, were isolated from the leaves of Impatiens parviflora DC. (Balsaminaceae). Their structures were determined based on a combination of one- and two-dimensional nuclear magnetic resonance (NMR) techniques, and mass spectrometry, as 3-O-{[beta-D-glucopyranosyl-(1 -> 3)+[(beta-D-glucopyranosyl-(1 -> 2)1}- beta-D-glucuronopyranoside 16-O-acetyl-3 beta,22 alpha,28-trihydroxy-olean-12-ene (named IPS-1) and 3-O-{[beta-D-glucopyranosyl-(1 -> 3)-]-[beta-D-glucopyranosyl-(1 2)])-[beta-D-glucuronopyranoside 3 beta,16 alpha,22 alpha,28-tetrahydroxy-olean-12-ene (named IPS-2). This is the first report on saponins in I. parviflora. Also, camelliagenin derivatives have been found in the Balsaminaceae famify for the first time. Novel saponin IPS-1 exhibited interesting cytotoxicity profile against human prostate an melanoma cancer cells with highest cytotoxic activity against A375 cell line (IC50 41.19 mu gfmL). Moreover, both isolated saponins were devoid of mutagenic potential against all tested strains. The obtained results allow to suggest that I. parviflora should be considered as a source of new promising bioactive metabolite. (C) 2016 Elsevier B.V. All rights reserved.