期刊
ADVANCED BIOSYSTEMS
卷 4, 期 11, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/adbi.202000123
关键词
calcium; dexamethasone; mesoporous silica nanoparticles; osteogenic differentiation; phosphate
资金
- Fundos Europeus Estruturais e de Investimento (FEEI)
- Programa Operacional Regional de Lisboa-FEDER [02/SAICT/2017]
- FundacAo para a Ciencia e a Tecnologia (FCT-Portugal) [UIDB/50011/2020, UIDB/00100/2020, PTDC/CTM-POL/3698/2014, PTDC/CTM-CTM/32444/2017 (02/SAICT/2017/032444)]
- COMPETE (FEDER) [UIDB/50011/2020, UIDB/00100/2020, PTDC/CTM-POL/3698/2014, PTDC/CTM-CTM/32444/2017 (02/SAICT/2017/032444)]
- FCT [FCT-PD/BD/114019/2015]
- Fundação para a Ciência e a Tecnologia [PTDC/CTM-POL/3698/2014] Funding Source: FCT
Bone regeneration requires the presence of specific factors to induce the differentiation of stem cells into osteoblasts. These factors induce osteogenesis by stimulating the expression of bone-related proteins, bone cell proliferation and differentiation. Herein, bioactive mesoporous silica nanoparticles are doped with calcium and phosphate ions while the porous network is loaded with dexamethasone (MSN-CaPDex). The bioactive MSN-CaPDex nanocarriers are prepared without affecting the narrow size distribution, pore structure, and morphology of the MSNs, while incorporating multi-stimuli, complementary ionic/biochemical bioactive mediators. The bioactive nanocarriers induce osteogenic differentiation of human bone marrow mesenchymal stem cells (hBM-MSCs) after a single-dose administration, and without the need for further soluble osteogenic factors, in contrast to the standard continuous stimulation provided by osteogenic medium. The hBM-MSCs exhibit several biomarkers of osteogenic differentiation, including alkaline phosphatase peaking at early time points, secretion of osteopontin and osteocalcin, and deposition of a calcium-rich matrix. Overall, by inducing the osteogenic differentiation of stem cells with a single-dose administration and without requiring repeated osteogenic supplementation, the newly synthesized multi-bioactive hybrid nanocarrier shows great potential for bone tissue engineering applications.
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