期刊
INDUSTRIAL CROPS AND PRODUCTS
卷 155, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.indcrop.2020.112837
关键词
Sugarcane; Hemicellulose hydrolysate; Molasses; Fed-batch fermentation; Butanol
资金
- National Council for Scientific and Technological Development (CNPq)
- Sao Paulo Research Foundation (FAPESP) [2015/20630-4, 2018/23983-3, 2018/25925-0]
- United States Department of Agriculture -NIFA Hatch grant
Butanol is a chemical and advanced biofuel whose fermentative production from hemicellulose hydrolysates is hindered by lignocellulose-derived microbial inhibitory compounds. In this work, Clostridium saccharoperbutylacetonicum DSM 14923 was not able to ferment a sugarcane hemicellulose hydrolysate supplemented with either laboratory-grade nutrients or sugarcane molasses. To circumvent this problem, the crude hemicellulose hydrolysate was fed, after 24 h, to a molasses fermentation containing 45 g/L total reducing sugars. Without the need of supplementing nutrients, the culture was able to co-ferment sucrose, glucose, fructose, and xylose (conversion of sucrose and xylose were 81 and 90 %, respectively) in a fermentation containing a diluted molasses-to-hydrolysate volume ratio of 3:1. Butanol yield (0.31 g/g) was remarkable, and butanol titer was 10.0 g/L after 72 h of fermentation. Therefore, this research demonstrated that sugarcane molasses a byproduct of the sugar industry rich in sucrose and nutrients can - be an efficient feedstock for enabling the production of butanol from sugarcane bagasse hemicellulose hydrolysate.
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