期刊
BIORESOURCE TECHNOLOGY
卷 316, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2020.123911
关键词
Lignocellulose; Co-substrate; Activated sludge; PHBV
资金
- Science Foundation of Tianjin Municipal Education Commission [2019ZD039]
- Natural Science Foundation of Tianjin City [18JCYBJC90100]
- Opening Project Fund of Key Laboratory of Biology and Genetic Resources of Rubber Tree, Ministry of Agriculture and Rural Affairs, P. R. China/State Key Laboratory Breeding Base of Cultivation Physiology for Tropical Crops/Danzhou Investigation Experim [RRI-KLOF202001]
The purpose of this study was to explore the potential of producing Poly(3-hydroxybutyrate-3-hydroxyvalerate) (PHBV) by mixed microbial culture (MMC) with lignocellulosic hydrolysates and acetate co-substrate as feedstock. The addition of co-substrate acetate led to the introduction of HV monomer into the polyhydroxyalkanoate (PHA), and the initial mixed sludge suspension (MLSS) increased with the increase of acetate. Almost 1.91-fold increase in the yield of PHA was achieved with limited nitrogen medium (the carbon to nitrogen ratio (C/N) was 33) compared to the normal nitrogen medium (C/N = 20). Limiting nitrogen source and micro alkaline culture environment was more conducive to the accumulation of PHBV. PHA production achieved to the highest value of about 2308.45 mg/L under the condition of optimized technology. Acidovorax was the dominant genus of all bioreactors using co-substrate. Further, utilizing lignocellulosic hydrolysate and acetate co-substrate as feedstock in mixed microbial culture was a promising approach in a low-cost large-scale PHA production.
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