期刊
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
卷 56, 期 31, 页码 8797-8805出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.iecr.7b01209
关键词
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资金
- National Natural Science Foundation of China [21376117]
- Jiangsu Natural Science Funds [BK20140043]
- Natural Science Foundation of Jiangsu Higher Education Institutions of China [14KJA150005]
- Qing Lan Project
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
In this paper, we designed and synthesized three benzothiazole-based fluorescent probes L-1, L-2, and L-3 for zinc ion detection. Among various metal ions, only the zinc ion exhibited fluorescence enhancement at 475 nm accompanied by the blue-shift emission wavelength in HEPES buffet solution containing probes. Through titration experiment, the detection limit of L-1 for zinc ion sensing was calculated to be as low as 7 nM, which showed a high sensitivity. Furthermore, the confocal laser scanning micrographs,of HeLa cells demonstrate good cell permeability of probe L-1 and selective detection of zinc ion in living cells. The L-1-Zn2+ complex was further used for pyrophosphate (PPi) sensing in HEPES buffer solution, the limit of detection was calculated to be as low as 60 nM. L-1-Zn2+ can monitor the enzyme catalyzed degradation process of PPi, thus providing a Meaningful way for tracking of zinc ion and pyrophosphate in biological systems.
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