Hydrothermal liquefaction of Nostoc ellipsosporum biomass grown in municipal wastewater under optimized conditions for bio-oil production

Microalgae offer numerous potential applications, however the industrial scale-up of algal technology still remains a challenge due to high production cost. Optimization of growth conditions and integration with waste streams can improve the economic viability of microalgal production systems. This study investigated on the optimal growth conditions of microalgae Nostoc ellipsosporum cultivated in municipal wastewater with the objective of achieving maximum biomass production, nutrient removal efficiency and bio-oil yield. The effect of light intensity, photoperiod, wavelength, aeration and growth media composition were studied. Different formulations of municipal wastewater blended with Fog's nutrient were used as growth medium. Optimization of growth conditions and acclimatization to wastewater enhanced the biomass yield of Nostoc ellipsosporum from 1.42 to 2.9 g L-1, achieving 87.59% of nitrogen removal and 88.31% of phosphate removal from wastewater. Furthermore, hydrothermal liquefaction of biomass produced bio-oil yield of 24.62% at 300 degrees C.