期刊
NANOSCALE
卷 12, 期 36, 页码 18692-18700出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/d0nr04581k
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资金
- Department of Science and Technology (DST, SERB) [ECR/2016/000401]
- Department of Biotechnology [BT/PR22067/NNT/28/1163/2016]
- UGC [2121310082]
- INST fellowship
- CSIR
Self-sorting is a spontaneous phenomenon that ensures the formation of complex yet ordered multicomponent systems and conceptualizes the design of artificial and orthogonally functional compartments. In the present study, we envisage chirality-mediated self-sorting in beta-amyloid-inspired minimalistic peptide amphiphile (C-10-l/d-VFFAKK)-based nanofibers. The fidelity and stereoselectivity of chiral self-sorting was ascertained by Forster resonance energy transfer (FRET) by the judicious choice of a pyrene (Py)-hydroxy coumarin (HOCou) donor-acceptor pair tethered to the peptide sequences. Seed-promoted elongation of the homochiral peptide amphiphiles investigated by AFM image analyses and Thioflavin-T (ThT) binding study further validated the chiral recognition of thel/dpeptide nanofibers. Moreover, direct visualization of the chirality-driven self-sorted nanofibers is reported using super-resolution microscopy that exhibits enantioselective enzymatic degradation forl-peptide fibers. Such enantioselective weakening of the hydrogels may be used for designing stimuli-responsive orthogonal compartments for delivery applications.
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