期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 323, 期 -, 页码 -出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2020.128691
关键词
Photothermal effect; OxTMB; Thermometer; Instrument-free analyses; Glucose
资金
- National Natural Science Foundation of China [21405124, 21175110]
- Fundamental Research Funds for the Central Universities [XDJK2020C033]
Although 3,3',5,5'-tetramethyl benzidine dihydrochloride (TMB) has been widely used as a chromogenic agent in colorimetric analysis, other characteristics of its one-electron oxidation product (oxTMB) have seldom been explored. OxTMB, which exhibits strong photothermal effect under near-infrared light, can transform the analysis signal into heat with a pen-style digital thermometer as the signal reader. Therefore, oxTMB has the potential for simple and instrument-free analyses. Based on the photothermal effect of oxTMB, a simple but sensitive photothermal assay is developed for glucose detection with a linear range of 5-300 mu M. The calculated limit of detection (LOD) for glucose was 0.13 mu M. Considering that thermometers are widely used in instrument-free analyses, the proposed photothermal method may provide a more affordable way to detect biomarkers associated with biological diseases.
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