期刊
CHEMICAL COMMUNICATIONS
卷 56, 期 77, 页码 11426-11428出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/d0cc04196c
关键词
-
资金
- NSF [MCB1615731, MCB1817675]
We report a new modular strategy to assemble dCas9-guided enzyme cascades by employing orthogonal post-translation chemistry. Two orthogonal SpyCatcher and SnoopCatcher pairs were used for the one-pot enzyme bioconjugation onto two different dCas9 proteins to enable their guided assembly onto a DNA scaffold. The resulting two-component cellulosomes exhibited 2.8-fold higher reducing sugar production over unassembled enzymes. This platform retains the high binding affinity afforded by dCas9 proteins for easy control over enzyme assembly while offering the flexibility for bothin vivoandin vitroassembly of a wide array of enzyme cascades with minimal optimization.
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