期刊
INDIAN JOURNAL OF ANIMAL RESEARCH
卷 53, 期 1, 页码 129-135出版社
AGRICULTURAL RESEARCH COMMUNICATION CENTRE
DOI: 10.18805/ijar.B-732
关键词
Histopathology; Lumpy skin disease; PCR; Sequencing
Lumpy skin disease (LSD) is a pox disease of cattle characterised by fever, nodules on the skin, mucous membranes and internal organs, emaciation, enlarged lymph nodes, oedema of the skin, and sometimes death. A disease form was observed in cattle in the Southeast Anatolia in 2013 and 2014 and suspected for LSD suspected by clinical examination. In this study, LSD has been defined by molecular virologic and histopathologic tests. A necropsy was undertaken and tissue samples were taken. Also, blood and tissue samples with biopsy punches were taken from animals having symptoms. Tissue and blood samples were examined molecularly and histopathologically while tissue samples were examined histopathologically. Positive amplification was detected by Polymerase Chain Reaction and confirmed by sequencing in the extracted DNA's derived from tissues. Tissue samples were compatible with LSD histopathologically. The first official notification of LSD in Sanliurfa province of the Southeast of Turkey is determined. As a result, LSD could be treated northern territories in suitable climate conditions.
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