期刊
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
卷 53, 期 7, 页码 646-650出版社
SPRINGER
DOI: 10.1007/s11626-017-0166-x
关键词
Mythimna separata; Cell clone strain; Autographa californica multiple nucleopolyhedrovirus (AcMNPV); Mythimna separata nucleopolyhedrovirus (MsNPV)
资金
- State Key Laboratory of Integrated Management of Pest Insects and Rodents [ChineseIPM1602]
- Key Research and Development Program in Shandong Province [2015GNC111003]
- Wheat Industrial I & E Team of Qingdao Modern Agriculture Industrial Technology System [QDAIET-16-01-06]
In this study, we describe a cell line, Ms-10C, cloned from the line QAU-Ms-E-10 (simplified Ms-10), an embryonic line from Mythimna separata. The cloned cell line was significantly more sensitive to nucleopolyhedrovirus (NPV). Ms-10C cells were mainly spherical with a diameter of 14.42 +/- 2.23 mu m. DNA amplification fingerprinting (DAF) confirmed the profile of PCR-amplified bands of the cloned cell line was consistent with those of the parental cell line, Ms-10. The sequencing result of the mitochondrial cytochrome c oxidase I (mtCO I) fragment confirmed that the amplified 636-bps mtCOI fragment was 100% identical to that of M. separata. Its chromosomes exhibited the typical characters of lepidopteran cell lines. Its population doubling time was 42.2 h at 27 degrees C. Ms-10C was more sensitive than Ms-10 to both Autographa californica multiple nucleopolyhedrovirus (AcMNPV) and M. separata nucleopolyhedrovirus (MsNPV). At 4 d post infection, the infection rates of two viruses reached 94.2 and 92.3%, respectively. The availability of this cell clone strain will provide a useful tool for the basic research on nucleopolyhedrovirus and for potential application in expression of recombinant proteins with baculovirus expression vector system.
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