期刊
CURRENT BIOLOGY
卷 25, 期 17, 页码 2300-2306出版社
CELL PRESS
DOI: 10.1016/j.cub.2015.07.030
关键词
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资金
- German Academic Exchange Service
- Higher Education Commission, Pakistan (DAAD-HEC)
- German Research Foundation (DFG) [HO3983/7-1, INST 20876/127-1 FUGG]
- Marie Curie-IEF [PIEF-GA-2012-331812]
- Max Planck Society
- University of Oxford
- European Research Council [616449]
- COST [CM1004]
- Biotechnology and Biological Science Research Council (BBSRC)
- Gatsby Foundation
- Dutch Scientific Organization (NWO ALW-VIDI) [864.10.004]
Rcr3 and Pip1 are paralogous secreted papain-like proteases of tomato. Both proteases are inhibited by Avr2 from the fungal pathogen Cladosporium fulvum, but only Rcr3 acts as a co-receptor for Avr2 recognition by the tomato Cf-2 immune receptor [1-4]. Here, we show that Pip1-depleted tomato plants are hyper-susceptible to fungal, bacterial, and oomycete plant pathogens, demonstrating that Pip1 is an important broad-range immune protease. By contrast, in the absence of Cf-2, Rcr3 depletion does not affect fungal and bacterial infection levels but causes increased susceptibility only to the oomycete pathogen Phytophthora infestans. Rcr3 and Pip1 reside on a genetic locus that evolved over 36 million years ago. These proteins differ in surface-exposed residues outside the substrate-binding groove, and Pip1 is 5- to 10-fold more abundant than Rcr3. We propose a model in which Rcr3 and Pip1 diverged functionally upon gene duplication, possibly driven by an arms race with pathogen-derived inhibitors or by coevolution with the Cf-2 immune receptor detecting inhibitors of Rcr3, but not of Pip1.
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