期刊
BIOFABRICATION
卷 12, 期 4, 页码 -出版社
IOP Publishing Ltd
DOI: 10.1088/1758-5090/abafc6
关键词
EC-SMC co-culture; coaxial printing; double-layer; free-standing blood vessel; without ultraviolet illumination
资金
- Ministry of Science and ICT, Republic of Korea [NRF-2020R1F1A1075779]
Human umbilical vein endothelial cells (HUVECs) and human aortic smooth muscle cells (HASMCs) were coaxially and continuously extruded without ultraviolet illumination using a microfluidic-based nozzle. Type I collagen (3 mg ml(-1)) containing HUVECs and a crosslinking reagent (100 mM CaCl2) were supplied as the core material. A mixture of 3 mg ml(-1)of type I collagen (25%) and 1.8% weight volume(-1)of sodium alginate (75%) was provided as the shell layer material surrounding the core material. The HUVECs were well proliferated at the core and reshaped into a monolayer formation along the axial direction of the scaffold. The HASMCs showed more than 90% cell viability in the shell layer. Fluorescent beads were passed through the inside channel of the scaffold with the HUVEC core and HASMC shell using an in-house connector. This double-layered scaffold showed higher angiogenesis in growth factor-free medium than the scaffold with only a HUVEC core. The HASMCs in the shell layer affected angiogenesis, extracellular matrix secretion, and outer diameter. The proposed technique could be applied to three-dimensional bioprinting for the production of high-volume vascularised tissue.
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