期刊
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
卷 170, 期 -, 页码 -出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.pestbp.2020.104705
关键词
Magnolia officinalis; Honokiol; Antifungal activity; Rhizoctonia solani
资金
- National Key Research and Development Program of China [2017YFD0201404]
- National Natural Science Foundation of China [21877056, 21672092]
- Key Program for international S&T cooperation projects of China Gansu Province [18YF1WA115]
Magnolia officinalis, as a well-known herb worldwide, has been widely used to treat multiple diseases for a long time. In this study, the petroleum ether extract from M. officinalis showed effective antifungal activity against seven plant pathogens (particularly against R. solani with an inhibition rate of 100.00% at 250 mu g/mL). Honokiol and magnolol, isolated by the bioassay-guided method, exhibited greater antifungal activity than tebuconazole (EC50 = 3.07 mu g/mL, p <= 0.001) against R. solani, which EC50 values were 2.18 mu g/mL and 3.48 mu g/mL, respectively. We used transcriptomics to explore the mechanism of action of honokiol against R. solani. Results indicated that honokiol may exert antifungal effects by blocking the oxidative phosphorylation metabolic pathway. Further studies indicated that honokiol induced ROS overproduction, disrupted the mitochondrial function, affected respiration, and blocked the TCA cycle, which eventually inhibited ATP production. Besides, honokiol also damaged cell membranes and caused morphological changes. This study demonstrated that the lignans isolated from M. officinalis possess the potential to be developed as botanical fungicides.
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