期刊
ELECTROCHIMICA ACTA
卷 359, 期 -, 页码 -出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.electacta.2020.136951
关键词
Alkaline phosphatase; Carbon microelectrodes; 3D cell cultures; Square wave voltammetry
资金
- DTU Nanotech
- DTU Nanolab
- Young Investigator Programme of the Villum Foundation [9301]
In bioelectrochemistry, cells are typically cultured in monolayers on 2D electrodes and methods such as electrochemical impedance spectroscopy (EIS) or voltammetry are used to detect changes in the cell population or responses to external stimuli. Here, we implement an electrochemical assay for the in situ monitoring of alkaline phosphatase (ALP) activity of Saos-2 cells cultured in a 3D gelatin hydrogel matrix with a thickness of 300 mu m deposited on pyrolytic carbon electrodes. After cell seeding, ALP activity for cells in the hydrogel was higher compared to 2D cell cultures probably due to unrestricted access to the enzyme on the complete cell membrane. Gradual decrease of cell proliferation, maturation and spheroid formation in the 3D cell culture resulted in slower increase and eventually a decrease in ALP activity over time. Finally, 3D microstructured electrodes and 3D carbon pillar microelectrodes with a height of 225 mu m were fabricated and applied for ALP detection directly in the 3D cell cultures. This resulted in higher electrochemical signals compared to 2D electrode configurations due to increased electrode surface area and penetration of the microelectrodes into the cell-laden hydrogel. (c) 2020 Elsevier Ltd. All rights reserved.
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