期刊
IMMUNOLOGICAL INVESTIGATIONS
卷 47, 期 1, 页码 1-17出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/08820139.2017.1367008
关键词
Autoreactive T cells; experimental autoimmune encephalomyelitis; multiple sclerosis; myelin oligodendrocyte protein
类别
资金
- National Natural Science Foundation of China [81172823, 81372448]
- Science & Technology Support Program of Jiangsu Province [BE2012739]
- Fundamental Research Funds for the Central Universities of China
- Post-graduate Research & Innovation Program of Jiangsu province [KYCX17-0164]
Background: Mouse experimental autoimmune encephalomyelitis (EAE) is widely used model of multiple sclerosis (MS). The role of autoreactive CD4(+) and CD8(+) T cells in the development of mouse EAE has been demonstrated. However, little information is available about the relation between the frequency and reactivity of myelin antigen-reactive CD4(+) and CD8(+) T cells in secondary lymphoid organs and their relevance with the inflammation and pathological lesion of CNS during the course of EAE mouse model.Methods: In this study, an EAE model with a clinical course containing acute onset, peak and chronic remission stages was established in C57BL/6J mice by myelin oligodendrocyte protein (MOG)(35-55) peptide immunization, and followed by the monitoring of clinical and pathological parameters and autoreactive T cells at different stages during the course.Results: The dynamic changes of inflammatory infiltration, myelin loss, and astrocyte proliferation in brain and spinal cord were highly consistent with clinical severity observed in EAE course. However, the frequencies of both MOG-specific CD4(+) and CD8(+) T cells in secondary lymphoid organs presented different dynamic trends from the IFN- production by MOG-reactive T cells. Meanwhile, the IL-17 production by MOG-reactive CD4(+) T cells was consistent with the proliferation of MOG-specific CD4(+) T cells.Conclusions: Both CD4(+) and CD8(+) T cells were most sensitive to MOG antigen stimulation for IFN- production during the early stage of EAE, but then rapidly lost the function despite their vigorous proliferation at the peak stage and later.
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