Decolourisation of dyes in greywater by mycoremediation and mycosorption process of fungi from peatland; primary study

The current study investigated the potential of fungi from peatland for decolorizing dyes in the artificial greywater as a function of oxidative enzymes which included laccase (Lac), manganese peroxidase (MnP), lignin peroxidase (LiP). The fungal isolates were obtained from the peatland on potato dextrose agar (PDA) and purified using single spore technique. Remazol Brilliant Blue R (RBBR), Methylene blue (MB) and Congo red (CR) were used as models for detecting the applicability of the fungal enzyme to decolorizing the dyes. The screening of fungal isolates for the decolourisation of RBBR, MB and CR were investigated using plate assay and liquid-phase assays. The results revealed the fungal isolates varied in their ability to produce oxidative enzymes dependent on the production medium. However, the decolourisation of RBBR in the PD broth medium ranged from 17.96 to 44.89% after 7 days, while ranged from 55.98 to 99.99% in artificial greywater after 15 days of the incubation period. The fungal isolates exhibited also differences in the production of oxidative enzyme. The maximum production of Lac in artificial greywater was recorded by Cochliobolus sp. No. 403 while the highest production of MnP and LiP was noted by Trichoderma sp. No. 102, and Aspergillus sp. No. 506, respectively. However, Aspergillus sp. No. 605 was used for further studies, because the fungus exhibited ability to produce Lac, MnP and LiP enzymes simultaneously. It can be concluded that the fungal isolates obtained from the peatland has a potential to decolorize the dyes in the artificial greywater. (c) 2019 Elsevier Ltd. All rights reserved. Selection and Peer-review under responsibility of the scientific committee of the 4th International Conference on Green Chemical Engineering and Technology: Materials Science.