Foliar application of green nanoparticles in Annona muricata L. plants and their effects in physiological and biochemical parameters

The objective of this study was to evaluate the changes of green metallic nanoparticles on biochemical and physiological effects in Annona muricata L., plants. Changes in proline, phenolic compounds, antioxidants enzyme, phenylalanine ammonia lyase (PAL) activity and phytohormones in A. muricata leaves exposed to Ag, Cu and Cu/Ag phytonanoparticles were evaluated up to 120 h. The results showed that the foliar application of green metallic nanoparticles at 250, 500, 750 and 1000 ppm caused changes significantly in flavonoids, anthocyanins, phenylalanine ammonia lyase and pemxidase activities in A. muricata leaves after 120 h exposure. The foliar application of green nanoparticles stimulates the enhancement of the antioxidant protection system that protect at A. muricata against oxidative stress caused by reactive oxygen species (ROS). Our results showed that values of gibberelins, auxins and proline depended of nanoparticle concentration and composition.