期刊
JOURNAL OF MOLECULAR CELL BIOLOGY
卷 12, 期 7, 页码 530-542出版社
OXFORD UNIV PRESS
DOI: 10.1093/jmcb/mjz103
关键词
primordial germ cell; miR-202-5p; Cdc42se1; Cdc42; migration
类别
资金
- National Natural Science Foundation of China [31771587, 31970535]
- Fundamental Research Funds for the Central Universities [17lgpy66]
- Pearl River S&T Nova Program of Guangzhou [201806010047]
- Science and Technology Planning Project of Guangdong Province [2017A030303010]
- Guangdong Natural Science Foundation [2015A030308012]
In many lower animals, germ cell formation, migration, and maintenance depend on maternally provided determinants in germ plasm. In zebrafish, these processes have been extensively studied in terms of RNA-binding proteins and other coding genes. The role of small non-coding RNAs in the regulation of primordial germ cell (PGC) development remains largely unknown and poorly investigated, even though growing interests for the importance of miRNAs involved in a wide variety of biological processes. Here, we reported the role and mechanism of the germ plasm-specific miRNA miR-202-5p in PGC migration: (i) both maternal loss and knockdown of miR-202-5p impaired PGC migration indicated by the mislocalization and reduced number of PGCs; (ii) cdc42se1 was a direct target gene of miR-202-5p, and overexpression of Cdc42se1 in PGCs caused PGC migration defects similar to those observed in loss of miR-202-5p mutants; (iii) Cdc42se1 not only interacted with Cdc42 but also inhibited cdc42 transcription, and overexpression of Cdc42 could rescue PGC migration defects in Cdc42se1 overexpressed embryos. Thus, miR-202-5p regulates PGC migration by directly targeting and repressing Cdc42se1 to protect the expression of Cdc42, which interactswith actin to direct PGC migration.
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