期刊
IMMUNITY
卷 47, 期 1, 页码 183-+出版社
CELL PRESS
DOI: 10.1016/j.immuni.2017.06.017
关键词
-
类别
资金
- Singapore Immunology Network
- National Research Foundation Singapore [NRF-NRFI2017-02]
- Strategic Positioning Fund for Genetic Orphan Diseases [SPF2012/005]
- Joint Council Office Project grant from AstarSTAR, Singapore [1431AFG122]
- Singapore National Medical Research Council [NMRC/CBRG/0047/2013]
- A*STAR Singapore Young Investigator Grant (BMRC YIG grant) [13/1/16/YA/009]
- ERC [616080]
- European Research Council (ERC) [616080] Funding Source: European Research Council (ERC)
Tissue macrophages arise during embryogenesis from yolk-sac (YS) progenitors that give rise to primitive YS macrophages. Until recently, it has been impossible to isolate or derive sufficient numbers of YS-derived macrophages for further study, but data now suggest that induced pluripotent stem cells (iPSCs) can be driven to undergo a process reminiscent of YS-hematopoiesis in vitro. We asked whether iPSC-derived primitive macrophages (iMacs) can terminally differentiate into specialized macrophages with the help of growth factors and organ-specific cues. Co-culturing human or murine iMacs with iPSC-derived neurons promoted differentiation into microglia-like cells in vitro. Furthermore, murine iMacs differentiated in vivo into microglia after injection into the brain and into functional alveolar macrophages after engraftment in the lung. Finally, iPSCs from a patient with familial Mediterranean fever differentiated into iMacs with pro-inflammatory characteristics, mimicking the disease phenotype. Altogether, iMacs constitute a source of tissue-resident macrophage precursors that can be used for biological, pathophysiological, and therapeutic studies.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据