Overexpression of JcWRKY2 confers increased resistance towards Macrophomina phaseolina in transgenic tobacco

WRKY proteins are plant-specific transcription factors (TFs), and form one of the largest families and are involved in plant development and responses to stress. The salicylic acid (SA) responsive WRKY family auto or cross-regulate the defence stress signalling pathways. In this study, we functionally validated the role of JcWRKY2 gene from biofuel crop Jatropha curcas towards improving resistance to tobacco transgenic against charcoal rot causing necrotrophic fungus, Macrophomina phaseolina. The microscopic studies revealed that JcWRKY2 participated in preventing the spread of infection in transgenic. The generation of H2O2 during M. phaseolina and combinatorial stress in transgenic induces the expression and activity of antioxidant enzymes. The transcript expression of SA biosynthetic (NtICS1) gene, pathogenesis-related (NtPR-10) gene and antioxidative enzymes (NtCAT1 and NtSOD) gene revealed that JcWRKY2 transgenic play a role in SA-mediated, antioxidative enzymes regulation during biotic challenges. The study highlights the potential of JcWRKY2 as an important regulator for plant biotic stress responses through the SA-dependent pathway.