期刊
IET NANOBIOTECHNOLOGY
卷 11, 期 8, 页码 1027-1034出版社
WILEY
DOI: 10.1049/iet-nbt.2017.0017
关键词
antibacterial activity; nanoparticles; silver; nanomedicine; wounds; microorganisms; X-ray diffraction; ultraviolet spectra; visible spectra; Fourier transform infrared spectra; transmission electron microscopy; phyto-assisted synthesis; biofunctionalised silver nanoparticles; antioxidant antimicrobial wound healing activities; silver nanoparticle biosynthesis; aqueous leaf extract; Ardisia solanacea; silver nitrate; UV-visible spectroscopy; dynamic light scattering; Fourier transform infra-red spectroscopy; X-ray diffraction; electron microscopy; attenuated total reflection Fourier transform infra-red spectroscopy; dilution method; Gram-positive bacteria; Gram-negative bacteria; radical scavenging method; Pseudomonas aeruginosa; Trichophyton mentagrophytes; Bacillus subtilis; Candida kruseii; BJ-5Ta normal fibroblast cell line; SEM; alcohols; aldehydes; flavonoids; phenols; nitro compounds; Ag
Bio- synthesis of silver nanoparticles (AgNPs) was made by using the aqueous leaf extract of Ardisia solanacea. Rapid formation of AgNPs was observed from silver nitrate upon treatment with the aqueous extract of A. solanacea leaf. The formation and stability of the AgNPs in the colloidal solution were monitored by UV-visible spectrophotometer. The mean particle diameter of AgNPs was calculated from the DLS with an average size approximate to 4nm and approximate to 65nm. ATR-FTIR spectroscopy confirmed the presence of alcohols, aldehydes, flavonoids, phenols and nitro compounds in the leaf which act as the stabilizing agent. Antimicrobial activity of the synthesized AgNPs was performed using agar well diffusion and broth dilution method against the Gram-positive and Gram-negative bacteria. Further, robust anti-oxidative potential was evaluated by DPPH assay. The highest antimicrobial activity of synthesized AgNPs was found against Pseudomonas aeruginosa (28.2 +/- 0.52mm) whereas moderate activity was found against Bacillus subtilis (16.1 +/- 0.76), Candida kruseii (13.0 +/- 1.0), and Trichophyton mentagrophytes (12.6 +/- 1.52). Moreover, the potential wound healing activity was observed against the BJ-5Ta normal fibroblast cell line. Current research revealed that A. solanacea was found to be a suitable source for the green synthesis of silver nanoparticles.
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