期刊
INFECTION GENETICS AND EVOLUTION
卷 85, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.meegid.2020.104438
关键词
Coronavirus; SARS-CoV; SARS-CoV-2; Bioinformatics; Ferret (Mustela putorius furo)
资金
- Human Biobank, Research Center of Clinical Medicine, National Cheng Kung University Hospital
- Ministry of Science and Technology (MOST) of Taiwan [MOST105-2325-B-006-003, MOST 108-2314-B-006-082, MOST 1082320-B-041 -002, MOST109-2320-B-038-009-MY2]
- National Cheng Kung University Hospital [NCKUH-10601002]
- Kaohsiung Medical University Hospital [KMUH108-8R72]
- Taipei Medical University [TMU-108-AE1-B16]
Coronaviruses (CoVs) consist of six strains, and the severe acute respiratory syndrome coronavirus (SARS-CoV), newly found coronavirus (SARS-CoV-2) has rapidly spread leading to a global outbreak. The ferret (Mustela putorius furo) serves as a useful animal model for studying SARS-CoV/SARS-CoV-2 infection and developing therapeutic strategies. A holistic approach for distinguishing differences in gene signatures during disease progression is lacking. The present study discovered gene expression profiles of short-term (3 days) and longterm (14 days) ferret models after SARS-CoV/SARS-CoV-2 infection using a bioinformatics approach. Through Gene Ontology (GO) and MetaCore analyses, we found that the development of stemness signaling was related to short-term SARS-CoV/SARS-CoV-2 infection. In contrast, pathways involving extracellular matrix and immune responses were associated with long-term SARS-CoV/SARS-CoV-2 infection. Some highly expressed genes in both shortand long-term models played a crucial role in the progression of SARS-CoV/SARS-CoV-2 infection, including DPP4, BMP2, NFIA, AXIN2, DAAM1, ZNF608, ME1, MGLL, LGR4, ABHD6, and ACADM. Meanwhile, we revealed that metabolic, glucocorticoid, and reactive oxygen species-associated networks were enriched in both shortand long-term infection models. The present study showed alterations in gene expressions from short-term to long-term SARS-CoV/SARS-CoV-2 infection. The current result provides an explanation of the pathophysiology for post-infectious sequelae and potential targets for treatment.
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