4.8 Article

Assembly of the algal CO2-fixing organelle, the pyrenoid, is guided by a Rubisco-binding motif

期刊

SCIENCE ADVANCES
卷 6, 期 46, 页码 -

出版社

AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/sciadv.abd2408

关键词

-

资金

  1. NSF [IOS-1359682, MCB-1935444]
  2. NIH [DP2-GM-119137]
  3. Simons Foundation
  4. Howard Hughes Medical Institute [55108535]
  5. UK Biotechnology and Biological Sciences Research Council [BB/R001014/1]
  6. BBSRC [BB/R506485/1, BB/S015337/1, BB/R001014/1] Funding Source: UKRI

向作者/读者索取更多资源

Approximately one-third of the Earth's photosynthetic CO2 assimilation occurs in a pyrenoid, an organelle containing the CO2-fixing enzyme Rubisco. How constituent proteins are recruited to the pyrenoid and how the organelle's subcompartments-membrane tubules, a surrounding phase-separated Rubisco matrix, and a peripheral starch sheath-are held together is unknown. Using the model alga Chlamydomonas reinhardtii, we found that pyrenoid proteins share a sequence motif. We show that the motif is necessary and sufficient to target proteins to the pyrenoid and that the motif binds to Rubisco, suggesting a mechanism for targeting. The presence of the Rubisco-binding motif on proteins that localize to the tubules and on proteins that localize to the matrix-starch sheath interface suggests that the motif holds the pyrenoid's three subcompartments together. Our findings advance our understanding of pyrenoid biogenesis and illustrate how a single protein motif can underlie the architecture of a complex multilayered phase-separated organelle.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.8
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据