期刊
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷 532, 期 4, 页码 505-512出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2020.08.037
关键词
Trehalose liposome; Apoptosis; NF kappa B; Breast cancer; Therapeutic effects
资金
- Ministry of Education, Science, and Culture of Japan [17K01383, 17K05944]
- Grants-in-Aid for Scientific Research [17K01383, 17K05944] Funding Source: KAKEN
The inhibitory effects of trehalose liposomes (TL) comprising L-alpha-dimyristoylphosphatidylcholine (DMPC) and alpha-D-glucopyranosyl-alpha-D-glucopyranoside monomyritate (TreC14) were investigated on breast cancer MDA-MB-453 cells in vitro and in vivo. The IC50 values of TL for MDA-MB-453 cells were remarkably lower than those of DMPC liposomes. The inhibitory effects of TL on the proliferation of MDA-MB-453 cells mediated via apoptosis induction were observed following their accumulation on MDA-MB-453 cell membranes. The membrane fluidity of MDA-MB-453 cells increased after TL treatment, as evident from a fluorescence depolarization assay. TL induced the apoptosis of MDA-MB-453 cells through caspase activation and mitochondrial membrane potential reduction, and suppressed the nuclear factor kappa B activity. A remarkable reduction in tumor volume was observed in a human breast cancer mouse model topically treated with TL. Induction of apoptosis was evident in TL-treated breast cancer tumors of mice using the TUNEL assay. (C) 2020 Elsevier Inc. All rights reserved.
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