期刊
THERANOSTICS
卷 10, 期 25, 页码 11794-11819出版社
IVYSPRING INT PUBL
DOI: 10.7150/thno.44152
关键词
Triple transgenic mouse model of Alzheimer's disease; blood-brain barrier; focused ultrasound; microbubbles; behavioral tests
资金
- National Natural Science Foundation of China [81673134, 81871429, 31870825, 81971637]
- Training Program of the Major Research Plan of the National Natural Science Foundation of China [91859122]
- Shenzhen Science and Technology Planning Project [JCYJ20170412110026229]
- Shenzhen Key Medical Discipline Construction Fund [SZXK069]
- Sanming Project of Medicine in Shenzhen [SZSM201611090]
- Guangdong Provincial Key ST Program [2018B030336001]
- Guangdong Natural Science Foundation [2016A030313051]
- Medical Young Scientists Program of Shenzhen University
Alzheimer's disease (AD) is a progressive neurodegenerative disease manifested by cognitive impairment. As a unique approach to open the blood-brain barrier (BBB) noninvasively and temporarily, a growing number of studies showed that low-intensity focused ultrasound in combination with microbubbles (FUS/MB), in the absence of therapeutic agents, is capable of ameliorating amyloid or tau pathology, concurrent with improving memory deficits of AD animal models. However, the effects of FUS/MB on both the two pathologies simultaneously, as well as the memory behaviors, have not been reported so far. Methods: In this study, female triple transgenic AD (3xTg-AD) mice at eight months of age with both amyloid-beta (A beta) deposits and tau phosphorylation were treated by repeated FUS/MB in the unilateral hippocampus twice per week for six weeks. The memory behaviors were investigated by the Y maze, the Morris water maze and the step-down passive avoidance test following repeated FUS/MB treatments. Afterwards, the involvement of A beta and tau pathology were assessed by immunohistochemical analysis. Neuronal health and phagocytosis of A beta deposits by microglia in the hippocampus were examined by confocal microscopy. Further, hippocampal proteomic alterations were analyzed by employing two-dimensional fluorescence difference gel electrophoresis (2D-DIGE) combined with mass spectrometry. Results: The three independent memory tasks were indicative of evident learning and memory impairments in eight-month-old 3xTg-AD mice, which developed intraneuronal A beta, extracellular diffuse A beta deposits and phosphorylated tau in the hippocampus and amygdala. Following repeated FUS/MB treatments, significant improvement in learning and memory ability of the 3xTg-AD mice was achieved. Amelioration in both A beta deposits and phosphorylated tau in the sonicated hemisphere was induced in FUS/MB-treated 3xTg-AD mice. Albeit without increase in neuron density, enhancement in axonal neurofilaments emerged from the FUS/MB treatment. Confocal microscopy revealed activated microglia engulfing A beta deposits in the FUS/MB-treated hippocampus. Further, proteomic analysis revealed 20 differentially expressed proteins, associated with glycolysis, neuron projection, mitochondrial pathways, metabolic process and ubiquitin binding etc., in the hippocampus between FUS/MB-treated and sham-treated 3xTg-AD mice. Conclusions: Our findings reinforce the positive therapeutic effects on AD models with both A beta and tau pathology induced by FUS/MB-mediated BBB opening, further supporting the potential of this treatment regime for clinical applications.
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