期刊
PHYSIOLOGICAL REPORTS
卷 8, 期 19, 页码 -出版社
WILEY
DOI: 10.14814/phy2.14610
关键词
5/6 nephrectomy; chronic kidney disease; metaproteomics; microbiome; microbiota; resistant starch
类别
资金
- Center for Scientific Review [P20GM103429, P20GM121293, R01DK101034, R21AI146521]
Background: Resistant Starch (RS) improves CKD outcomes. In this report, we study how RS modulates host-microbiome interactions in CKD by measuring changes in the abundance of proteins and bacteria in the gut. In addition, we demonstrate RS-mediated reduction in CKD-induced kidney damage. Methods: Eight mice underwent 5/6 nephrectomy to induce CKD and eight served as healthy controls. CKD and Healthy (H) groups were further split into those receiving RS (CKDRS, n = 4; HRS, n = 4) and those on normal diet (CKD, n = 4, H, n = 4). Kidney injury was evaluated by measuring BUN/creatinine and by histopathological evaluation. Cecal contents were analyzed using mass spectrometry-based metaproteomics and de novo sequencing using PEAKS. All the data were analyzed using R/Bioconductor packages. Results: The 5/6 nephrectomy compromised kidney function as seen by an increase in BUN/creatinine compared to healthy groups. Histopathology of kidney sections showed reduced tubulointerstitial injury in the CKDRS versus CKD group; while no significant difference in BUN/creatinine was observed between the two CKD groups. Identified proteins point toward a higher population of butyrate-producing bacteria, reduced abundance of mucin-degrading bacteria in the RS fed groups, and to the downregulation of indole metabolism in CKD groups. Conclusion: RS slows the progression of chronic kidney disease. Resistant starch supplementation leads to active bacterial proliferation and the reduction of harmful bacterial metabolites.
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