期刊
FEMS MICROBIOLOGY REVIEWS
卷 44, 期 5, 页码 565-571出版社
OXFORD UNIV PRESS
DOI: 10.1093/femsre/fuaa022
关键词
bacterial stress response; gene regulation; phenotypic heterogeneity; single-cell imaging; microfluidics
类别
资金
- Sir Henry Dale Fellowship [206159/Z/17/Z]
- Wellcome Trust [206159/Z/17/B]
- Hugh Price Fellowship at Jesus College, Oxford
- Wellcome Trust [206159/Z/17/B, 206159/Z/17/Z] Funding Source: Wellcome Trust
Stress responses are crucial for bacteria to survive harmful conditions that they encounter in the environment. Although gene regulatory mechanisms underlying stress responses in bacteria have been thoroughly characterised for decades, recent advances in imaging technologies helped to uncover previously hidden dynamics and heterogeneity that become visible at the single-cell level. Despite the diversity of stress response mechanisms, certain dynamic regulatory features are frequently seen in single cells, such as pulses, delays, stress anticipation and memory effects. Often, these dynamics are highly variable across cells. While any individual cell may not achieve an optimal stress response, phenotypic diversity can provide a benefit at the population level. In this review, we highlight microscopy studies that offer novel insights into how bacteria sense stress, regulate protective mechanisms, cope with response delays and prepare for future environmental challenges. These studies showcase developments in the single-cell imaging toolbox including gene expression reporters, FRET, super-resolution microscopy and single-molecule tracking, as well as microfluidic techniques to manipulate cells and create defined stress conditions.
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