期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 68, 期 45, 页码 12738-12748出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.0c05308
关键词
food nutrition; phosphoproteins; electrochemiluminescence sensor; aminated nanotitanium dioxide; upconversion nanoparticles
资金
- National Key R&D Program of China [2016YFD0401202]
- open Project of the State Key Laboratory of Food Nutrition and Safety [SKLFNS-KF-202010]
Evaluation of the nutrition and determination of phosphoproteins is of great importance in different foods as aberrant phosphorylation changes many biological processes and can relate to health conditions. In this study, an ultrafast (5 min) and sensitive electrochemiluminescence (ECL) sensor was innovatively fabricated for the determination of phosphoproteins in foods on the basis of fluorescent probe NH2-TiO2/upconversion nanomaterials (UCNPs). Impressively, the ECL intensity of NH2-TiO2/UCNPs-rGO/GCE was remarkably enhanced by 29 times. Furthermore, the photoactive NH2-TiO2 layer provided not only specific selectivity but also a large surface area as well as an unprecedented photocatalytic activity for the NH2-TiO2 /UCNPs-rGO/GCE ECL sensor (TIECLS), which could serve as an identification element for trace phosphoproteins. Under optimal conditions, the TIECLS achieved a relatively low detection limit of 9.2 X 10(-5) mg/mL (S/N = 3). Practical application of this TIECLS was carried out in different food samples with satisfying results, which were validated by laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS).
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