期刊
ELIFE
卷 9, 期 -, 页码 -出版社
ELIFE SCIENCES PUBLICATIONS LTD
DOI: 10.7554/eLife.57681
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资金
- Cancer Prevention and Research Institute of Texas [RR160057]
- National Institutes of Health [R00 GM120386, R01HL068702, R33CA235254, R35GM133522, K25 CA204526, P30 CA142543, 1R01MH120131-01A1, 1R34NS121873, 5P30CA142543, R01GM110066]
- Damon Runyon Cancer Research Foundation [DFS-24-17]
- Chan Zuckerberg Initiative [HCA3-0000000196]
- Australian Research Council [FT190100516]
- Rebecca L. Cooper Medical Research Foundation [PG2018168]
- University of Queensland [RM2018002613]
- Company of Biologists [JCSTF1903138]
- Robert A. Welch Foundation [I-1950-20180324]
- Human Frontier Science Program [LT000911/2018C]
- Australian Research Council [FT190100516] Funding Source: Australian Research Council
We present an oblique plane microscope (OPM) that uses a bespoke glass-tipped tertiary objective to improve the resolution, field of view, and usability over previous variants. Owing to its high numerical aperture optics, this microscope achieves lateral and axial resolutions that are comparable to the square illumination mode of lattice light-sheet microscopy, but in a user friendly and versatile format. Given this performance, we demonstrate high-resolution imaging of clathrin-mediated endocytosis, vimentin, the endoplasmic reticulum, membrane dynamics, and Natural Killer-mediated cytotoxicity. Furthermore, we image biological phenomena that would be otherwise challenging or impossible to perform in a traditional light-sheet microscope geometry, including cell migration through confined spaces within a microfluidic device, subcellular photoactivation of Rac1, diffusion of cytoplasmic rheological tracers at a volumetric rate of 14 Hz, and large field of view imaging of neurons, developing embryos, and centimeter-scale tissue sections.
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