期刊
LAB ON A CHIP
卷 20, 期 21, 页码 4031-4042出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/d0lc00615g
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资金
- National Institutes of Health [R01HL144157]
- National Science Foundation [EFMA 1830941, CBET 1743662, PFI 1827535]
This work demonstrates a novel high-throughput (HT) microfluidics-enabled uninterrupted perfusion system (HT-mu UPS) and validates its use with chronic all-optical electrophysiology in human excitable cells. HT-mu UPS consists of a soft multichannel microfluidic plate cover which could button on a commercial HT 96-well plate. Herein, we demonstrate the manufacturing process of the system and its usages in acute and chronic all-optical electrophysiological studies of human induced pluripotent stem-cell-derived cardiomyocytes (iPSC-CM) and engineered excitable (spiking HEK) cells. HT-mu UPS perfusion maintained functional voltage and calcium responses in iPSC-CM and spiking HEK cells under spontaneous conditions and under optogenetic pacing. Long-term culture with HT-mu UPS improved cell viability and optogenetically-tracked calcium responses in spiking HEK cells. The simplicity of this design and its compatibility with HT all-optical electrophysiology can empower cell-based assays for personalized medicine using patient-derived cells.
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