期刊
PURINERGIC SIGNALLING
卷 16, 期 4, 页码 585-599出版社
SPRINGER
DOI: 10.1007/s11302-020-09752-9
关键词
Dihydromyricetin; Diabetic neuropathic pain; Depression; Hippocampal astrocyte; P2X(7) receptor
资金
- National Natural Science Foundation of China [81760152]
Activated astrocytes play a key role in diabetic neuropathic pain and depression. We aimed to assess the protective effects of dihydromyricetin (DHM) on primary hippocampal astrocytes cultured with high glucose (HG), substance P (SP), and corticosterone (CORT). Culturing with HG + SP + CORT resulted in damage to primary hippocampal astrocytes, which simulates the clinical damage caused by comorbidity of diabetic neuropathic pain and depression. Western blot, qPCR, and immunofluorescence analyses revealed that HG + SP + CORT increased P2X(7) receptor expression in primary hippocampal astrocytes, which was reversed by DHM treatment. Further, HG + SP + CORT elevated TNF-alpha, IL-1 beta, free Ca2+, and ERK1/2 phosphorylation levels, which was inhibited by DHM or P2X(7) shRNA treatment. Moreover, DHM significantly reduced the P2X(7) agonist-activated currents in HEK293 cells transfected with the P2X(7) receptor. These findings suggest that DHM can protect primary hippocampal astrocytes cultured with HG + SP + CORT from P2X(7) receptor-mediated damage. Culturing cells with HG + SP + CORT might be a viable cell model for cellular injury exploration of diabetic comorbid pain and depression.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据