期刊
CHEMICAL SCIENCE
卷 11, 期 40, 页码 11037-11041出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/d0sc03037f
关键词
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资金
- National Natural Science Foundation of China [21977044, 21907050, 21731004, 91953201]
- Natural Science Foundation of Jiangsu Province [BK20190282]
- Excellent Research Program of Nanjing University [ZYJH004]
Monitoring labile Zn(2+)homeostasis is of great importance for the study of physiological functions of Zn(2+)in biological systems. Here we report a novel ratiometric fluorescent Zn(2+)sensor, CPBT, which was constructed based on chelation-induced alteration of FRET efficiency. CPBT was readily cell membrane permeable and showed a slight preferential localization in the endoplasmic reticulum. With this sensor, 3D ratiometric Zn(2+)imaging was first realized in the head of zebra fish larvaeviaZ-stack mode. CPBT could track labile Zn(2+)in a large number of cells through ratiometric flow cytometric assay. More interestingly, both ratiometric fluorescence imaging and flow cytometric assay demonstrated that the labile Zn(2+)level in MCF-7 cells (cisplatin-sensitive) decreased while that in SKOV3 cells (cisplatin-insensitive) increased after cisplatin treatment, indicating that Zn(2+)may play an important role in cisplatin induced signaling pathways in these cancer cells.
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