期刊
CELL STRUCTURE AND FUNCTION
卷 45, 期 2, 页码 121-130出版社
JAPAN SOC CELL BIOLOGY
DOI: 10.1247/csf.20020
关键词
endocytosis; GluR2; AMPA; cortactin; MIN6
类别
资金
- Ministry of Education, Culture, Sports, Science and Technology of Japan [19H03225, 20K08591]
- Grants-in-Aid for Scientific Research [19H03225, 20K08591] Funding Source: KAKEN
The activity of AMPA-type glutamate receptor is involved in insulin release from pancreatic beta-cells. However, the mechanism and dynamics that underlie AMPA receptor-mediated insulin release in beta-cells is largely unknown. Here, we show that AMPA induces internalization of glutamate receptor 2/3 (GluR2/3), AMPA receptor subtype, in the mouse beta-cell line MIN6. Immunotluorescence experiments showed that GluR2/3 appeared as fine dots that were distributed throughout MIN6 cells. Intracellular GluR2/3 co-localized with AP2 and clathrin, markers for clathrin-coated pits and vesicles. Immunoelectron microscopy revealed that GluR2/3 was also localized at plasma membrane. Surface biotinylation and immunolluorescence measurements showed that addition of AMPA caused an approximate 1.8-fold increase in GluR2/3 internalization under low-glucose conditions. Furthermore, internalized GluR2 largely co-localized with EEA1, an early endosome marker. In addition, GluR2/3 co-immunoprecipitated with cortactin, a F-actin binding protein. Depletion of cortactin by RNAi in MIN6 cells altered the intracellular distribution of GluR2/3, suggesting that cortactin is involved in internalization of GluR2/3 in MIN6 cells. Taken together, our results suggest that pancreatic beta-cells adjust the amount of AMPA-type GluR2/3 on the cell surface to regulate the receptive capability of the cell for glutamate.
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