期刊
CHEM
卷 6, 期 11, 页码 2893-2901出版社
CELL PRESS
DOI: 10.1016/j.chempr.2020.10.010
关键词
-
资金
- National Research Foundation of Korea (NRF) - Korean Government (MSIP) [2012R1A3A2048814]
Enzymes play a key role in many bioprocesses associated with diseases, and observation of enzyme dynamics in living bodies has become meaningful for understanding enzyme function. Thus, in situ real-time fluorescence imaging can prove to be a powerful tool for monitoring enzyme dynamics, enabling detailed understanding of changes in the activity and function of enzymes. This perspective summarizes the existing methods for in situ real-time fluorescence imaging of enzymes in vivo and discusses remaining challenges. This perspective is intended to encourage chemists and chemical engineers to develop additional methods for the in situ real-time monitoring of enzymes in complex biosystems.
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