4.7 Article

Production of a tributyltin-binding protein 2 knockout mutant strain of Japanese medaka, Oryzias latipes

期刊

MARINE POLLUTION BULLETIN
卷 160, 期 -, 页码 -

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PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.marpolbul.2020.111601

关键词

Tributyltin-binding protein2 (TBT-bp2); Detoxification; Oryzias latipes; CRISPR/Cas9; Knockout

资金

  1. Japan Society for the Promotion of Science (JSPS) KAKENHI [JP18H02280]

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Tributyltin-binding proteins (TBT-bps), members of the lipocalin family, bind TBT in fish blood and are pre-sumed to contribute to detoxification of TBT. Recent studies have shown that many fish species have TBT-bp genes, and that these genes are induced by stresses such as exposure to chemicals or fish pathogenic bacteria. However, the function of TBT-bps, and the mechanisms of their induction and detoxification activity are still unclear. Here, towards elucidating the functions of TBT-bp2, we produced a TBT-bp2 knockout (TBT-bp2(-/-)) strain of Japanese medaka, Oryzias latipes, by using the CRISPR/Cas9 system. Gene expression of the mutated TBT-bp2 was reduced, and the cDNA sequencing and predicted protein structure suggested possible loss of function. However, the fish could be grown under normal conditions. Exposure of the TBT-bp2(-/-) strain of medaka to various stresses in future experiments is expected to contribute to our understanding of this novel detoxification system in aquatic organisms.

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